In this experiment, Alor major low molecular weight component of the commercially available Aloe (A-0451, Bar-kicking content Sigma Chemical Company, USA about 20%) and (B-6906 Alor, Bar-foot con...

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In this experiment, Alor major low molecular weight component of the commercially available Aloe (A-0451, Bar-kicking content Sigma Chemical Company, USA about 20%) and (B-6906 Alor, Bar-foot content. Approximately 97% of Sigma Chemical Company, USA) It was used as the control reagent. Components of the aloe Barr feet amount (%) is also shown. One.

 

Measurement of the dye leakage in the pancreas and the pancreas in the experiments increased the vascular permeability in mouse pancreas

 

Measurement of the dye leakage in the pancreas and the pancreas was performed according to the method described above (bestow et al., 1987). Ten-week-old female ICR mice and are divided into seven groups, it was fasted for 15 hours. In six of the 160 mg group it was administered kg (Boehringer Mannheim, Germany) intraperitoneally (SZ- treatment group) / SZ. In the rest of the group, PBS was administered only (non-treatment group). In addition, / 10g body weight of 100 to SZ group five (powder amount: about 20 mg) after the intraperitoneal administration of the SZ aloe component (of SZ + aloe group) was administered for 30 minutes. In the remaining groups were administered PBS (SZ + Automotive Group).

 

Then, after all the groups administered the weight of the 2% Evans Blue in 50 / 10g body in SZ vein 5.5 hour infusion. After 30 minutes (6 hours after administration SZ), animals are pulled out of the blood through the heart under ether anesthesia. By performing laparotomy under ether anesthesia to extract the pigment from the pancreas, the pancreas was removed and disconnect the pancreas.

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